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PEG is used in protoplast culture as
PEG is used in protoplast culture as
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The correct option is D Fusogen
Protoplasts fusion is a physical phenomenon. During fusion, two or more protoplasts come in contact and adhere with one another spontaneously or in presence of fusion inducing chemicals. An important aspect has been that incompatibility barriers do not exist during the cell fusion process at Interspecific, Intergeneric or even inter kingdom level. PEG induces protoplast aggregation and subsequent fusion. But the concentration and molecular weight of PEG are important with respect to fusion. A solution of 37.5% w/v PEG of molecular weight 1500 to 6000 aggregates mesophyll and cultured cell protoplasts during a 45 minutes incubation at room temperature. Fusion of protoplast takes place during slow elusion of PEG with liquid culture medium. Carrot protoplast can be fused by 28% PEG 1500 and fusion can be promoted by Ca++ ion at concentration of 3.5 mM. But higher concentration of Ca++ ion has been considered beneficial. In some studies, high PH/Ca++ and PEG method have been combined. The PEG method has been modified slightly to fuse higher plant protoplast as indicated below:
a) PEG is more effective when it is mixed with 10-15% dimethyl sulfoxide.
b) Addition of concanvalin A to PEG increases protoplast fusion frequency.
c) Sea water has been used alone or in combination with PEG to fuse protoplasts.
Protoplasts fusion is a physical phenomenon. During fusion, two or more protoplasts come in contact and adhere with one another spontaneously or in presence of fusion inducing chemicals. An important aspect has been that incompatibility barriers do not exist during the cell fusion process at Interspecific, Intergeneric or even inter kingdom level. PEG induces protoplast aggregation and subsequent fusion. But the concentration and molecular weight of PEG are important with respect to fusion. A solution of 37.5% w/v PEG of molecular weight 1500 to 6000 aggregates mesophyll and cultured cell protoplasts during a 45 minutes incubation at room temperature. Fusion of protoplast takes place during slow elusion of PEG with liquid culture medium. Carrot protoplast can be fused by 28% PEG 1500 and fusion can be promoted by Ca++ ion at concentration of 3.5 mM. But higher concentration of Ca++ ion has been considered beneficial. In some studies, high PH/Ca++ and PEG method have been combined. The PEG method has been modified slightly to fuse higher plant protoplast as indicated below:
a) PEG is more effective when it is mixed with 10-15% dimethyl sulfoxide.
b) Addition of concanvalin A to PEG increases protoplast fusion frequency.
c) Sea water has been used alone or in combination with PEG to fuse protoplasts.
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