The correct option is A Cell wall
The last stage of peptidoglycan synthesis, present in cell wall, is inhibited by β-lactam antibiotics (penicillin) and glycopeptide (vancomycin) antibiotics.
The site of action of both the antibiotics happens to be the cell wall especially that of the Gram-positive bacteria.
The members of genus Mycoplasma lack cell wall and hence, are not affected by these antibiotics.
Thus, the correct answer is option (A).