Question

Plasmid can be considered as a suitable cloning vector.

(I) It can be readily isolated from cells
(II) It possesses a single recognition site for one restriction enzymes
(III) It should have a high molecular weight
(IV) Plasmids and bacteriophages have the ability to replicate within bacterial cells

• A. I, II, III
• B. I, II, III, IV
• C. II, III, IV
• D. I, and III

Answer 1

Answer: (B)

I, II, III, IV

The plasmids are the small extra-chromosomal DNA present in the bacteria. These plasmids are commonly used as a cloning vector. The desired gene fragment can be inserted in the plasmid and then introduced into the host. The plasmid is selected on the basis of certain features. The plasmid can be isolated from the bacterial cells very easily. There should be single restriction sites for the restriction enzyme. This would allow proper integration of the gene without disintegration of the plasmid. The molecular weight of the plasmid should be high which would allow easy separation of the plasmid from the cellular debris. The plasmids and the bacteriophage DNA have the ori site. This is the site which helps in the initiation of replication.

Thus, the correct answer is option B.