Question

Plasmids useful in genetic engineering are cut at specific sites by

• A. Same enzymes that cut the portion of DNA to be inserted into it
• B. Hot alkaline solution
• C. Any hydrolytic enzymes
• D. Ligase enzyme

Answer 1

Answer: (A)

Same enzymes that cut the portion of DNA to be inserted into it

• Plasmids are the vectors that are used for the transfer of genes from the parent cell to the desired cell.
• If we cut plasmid DNA and foreign DNA with the same restriction enzyme both will produce fragments with the same complementary sticky ends.
• Foreign DNA and a plasmid having complementary ends can be easily ligated using DNA ligase.
  

So, the correct answer is option A.