Polyethylene glycol method is used for:

Energy production from sewage

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Gene transfer without a vector

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Biodiesel production

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Seedless fruit production

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Solution

The correct option is B Gene transfer without a vector
Polyethylene glycol mediated DNA uptake is a direct gene transfer method that utilizes the interaction between polyethylene glycol, naked DNA, salts and the protoplast membrane to effect transport of the DNA into the cytoplasm.

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