Question

Polyethylene glycol method is used for which of the following techniques?

• A. Gene transfer without a vector
• B. Biodiesel production
• C. Seedless fruit production
• D. Energy production from sewage

Answer 1

Answer: (A)

Gene transfer without a vector

Polyethylene glycol method is also called as PEG. Foreign DNA is introduced into protoplasts prepared protonemata. Regeneration rate of protoplasts is one of most critical factors for transformation efficiency. Protonemata grown for 3 to 6 days after subculture should be used for isolating protoplasts.