Polymerase chain reaction is useful in

Locating simple sequence repeats

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Locating variable number of tandem repeats

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Developing restriction maps

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Amplification of specific DNA segment.

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Solution

The correct option is D Amplification of specific DNA segment.
Polymerase chain reaction (PCR) is a method widely used in molecular biology to make many copies of a specific DNA segment. Using PCR, a single copy (or more) of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment.
So, the correct answer is 'Amplification of specific DNA segment.'

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