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Polymerase chain reaction technology (PCR - technique) is used for
Polymerase chain reaction technology (PCR - technique) is used for
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The correct option is D DNA amplification
PCR - technique amplifies the gene of interest in three steps namely, denaturation of target DNA (thermal cycle to separate the DNA strands), annealing of primers to the ssDNA and polymerization (extension of primer into complete DNA strand complementary to the template strand). After completion of one cycle of PCR, two copies of the target DNA are produced both of which serve as a template for the next PCR cycle and produce 4 copies. Hence, there is an exponential amplification of DNA copies. DNA repair is the process to minimize cell killing, mutations and replication errors in DNA. It fixes the errors that occurred in the DNA replication by mismatch repair, nucleotide excision repair, recombinational repair, etc., it does not amplify the DNA.DNA identification is done through DNA fingerprinting. It is a forensic technique that identifies the individuals by their DNA characteristics. A variable number tandem repeat (or VNTR) refers to the presence of a short nucleotide sequence as a tandem repeat on many chromosomes; it exhibits variations in length and number of repeats between individuals. VNTR is 10-100 bp long. This DNA polymorphism is revealed during DNA fingerprinting in creating a DNA profile of individuals. Restriction endonucleases are the enzymes that recognize specific sequences in the DNA and cleave the DNA into fragments. The specific sites are 4-6 bp long and are called restriction sites.
PCR - technique amplifies the gene of interest in three steps namely, denaturation of target DNA (thermal cycle to separate the DNA strands), annealing of primers to the ssDNA and polymerization (extension of primer into complete DNA strand complementary to the template strand).
After completion of one cycle of PCR, two copies of the target DNA are produced both of which serve as a template for the next PCR cycle and produce 4 copies. Hence, there is an exponential amplification of DNA copies.
DNA repair is the process to minimize cell killing, mutations and replication errors in DNA. It fixes the errors that occurred in the DNA replication by mismatch repair, nucleotide excision repair, recombinational repair, etc., it does not amplify the DNA.
DNA identification is done through DNA fingerprinting. It is a forensic technique that identifies the individuals by their DNA characteristics. A variable number tandem repeat (or VNTR) refers to the presence of a short nucleotide sequence as a tandem repeat on many chromosomes; it exhibits variations in length and number of repeats between individuals. VNTR is 10-100 bp long. This DNA polymorphism is revealed during DNA fingerprinting in creating a DNA profile of individuals. Restriction endonucleases are the enzymes that recognize specific sequences in the DNA and cleave the DNA into fragments. The specific sites are 4-6 bp long and are called restriction sites.
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