Question

Positional cloning refers to

• A. Using a selection procedure to clone a cDNA.
• B. Cloning a portion of gene using PCR.
• C. Isolating a gene by PCR using primers from another species.
• D. Mapping a gene to a chromosomal region and then identifying and cloning a genomic copy of the gene from the region.

Answer 1

The correct option is D Mapping a gene to a chromosomal region and then identifying and cloning a genomic copy of the gene from the region.

Positional cloning is the approach of choice for the identification of genetic mutations underlying the pathological development of disease with simple mendelian inheritance.