Assume that √5 - √3 = p/q (it's rational).
Multiple both sides by (√5 + √3).
(√5 - √3) (√5 + √3) = 5-3 = 2 = p/q * (√5 + √3)
(√5 + √3) = 2q/p, therefore √5 +√3 is rational = 2q/p
√5 - √3 = p/q
√5 +√3 = 2q/p
√5 = [(p/q) + (2q/p)]/2, a rational number.
But we know that √5 is IRRATIONAL (easily provable, let me know if you need the proof).
Therefore the assumption is wrong and √5 - √3 is irrational.