The correct option is B Third irreversible step of glycolysis
Pyruvate kinase enzyme catalyses third irreversible step of glycolysis. It is the the final step of glycolysis in which phosphoenolpyruvate converts into pyruvate with the help of the enzyme pyruvate kinase. This reaction involves the transfer of a phosphate group. The phosphate group attached to the 2′ carbon of the PEP is transferred to a molecule of ADP, yielding ATP. Hence option C is correct.