Rakshita followed the following procedure for staining the temporary mount of
leaf peel on slide.
(I) to put a single drop of stain on leaf peel and wash it with water.
(II) to put a single drop of glycerine on leaf peel .
(III) cover the leaf peel with coverslip.
(IV) observe the slide under microscope.
Which of the following gives the correct sequence of steps ?