Question

Read the following statements and choose the most appropriate option.
I. ____A____ are bombarded with high velocity micro-particles of ___B____ coated with DNA in a method known as biolistics.
II. Recombinant DNA is directly injected into the nucleus of an animal cell in the method called as___C______.

• A. A - Plant cells, B - gold, C - gene gun
• B. A - Plant cells, B - gold/ aluminium , C - electroporation
• C. A - Animal cells, B - aluminium/ tungsten, C - electroporation
• D. A - Plant cells, B - gold/ tungsten, C - microinjection

Answer 1

The correct option is D A - Plant cells, B - gold/ tungsten, C - microinjection

Biolistics, microinjection and electroporation are methods of direct gene delivery into the host cells.

Biolistics/ gene gun - Plant cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA. Gold or tungsten is used as they are inert/ non reactive in nature.

Microinjection - Recombinant DNA is directly injected into the nucleus of an animal cell.

Electroporation - It uses electrical fields to destabilise the cell membrane for some time. This allows the entry of normally impermeable DNA into the cell.