Question

Read the following statements and select the incorrect ones.

(i) When the transformed cells on agar plates containing ampicillin are spread, both transformed and untransformed cells will grow.

(ii) Restriction enzymes are used in isolation and separation of DNA from other macromolecules.

(iii) Downstream processing is one of the steps of rDNA technology.

(iv) Disarmed pathogen vectors are also used in the transfer of rDNA into the host.

• A. (ii) and (iii)
• B. (iii) and (iv)
• C. (i) and (iii)
• D. (i) and (ii)

Answer 1

Answer: (D)

(i) and (ii)

Restriction enzymes are used to cut DNA at specific sites. When the transformed cells on agar plates containing ampicillin are spread, only transformants will grow, untransformed recipient cells will die while downstream processing is one of the steps of rDNA technology and disarmed pathogen vectors are also used in the transfer of rDNA into the host.
So, the correct option is option D.