Question

Read the following statements carefully and select the correct option:
(A) Source of the restriction enzyme Hind III is E.coli
(B) In biolistic method of gene transfer, mircoparticles made up of gold or tungsten are coated with foreign DNA
(C) Micro-injection method for injecting recombinant DNA is used for animal cell
(D) Primers are chemically synthesized oligonucleotides that are complementary to the regions of DNA in PCR.
How many of the above statement are correct?

• A. Four
• B. Three
• C. Two
• D. One

Answer 1

The correct option is B Three

A)

Restriction enzymes, also called restriction endonuclease, is a protein that cuts DNA after recognizing a specific sequence of DNA. Hind III is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA via hydrolysis.

B)

In the bolistic method of gene transfer, a gene gun is used to transfer exogenous or foreign DNA in the cell. A droplet of tungsten powder and genetic material is a bullet. Gold is favored at the place of tungsten as it has better uniformity than tungsten and tungsten can be toxic to cells.

C)

Micro-injection method for injecting recombinant DNA is used to produce transgenic mice. Harvested oocytes of the mouse are injected with the genetic material and then implanted in the oviduct of a pseudopregnant animal.

D)

A primer is a short strand of RNA or DNA that serves as a starting point for DNA synthesis.In vitro lab techniques that use DNA polymerase, DNA. primers are used. They are usually short, chemically synthesized oligonucleotides, with a length of about twenty bases.

Therefore A, B, and D are correct statements. Answer is B.