Question

Read the passage and answer the following question.

Bacterial plasmid sequence: ATCCCA...1000 more nucleotides.......ATTAAGGCCTTACAGGACCCCGGAATGAGAGTAATGGGCTATTCAGGTA... 2000 more nucleotides.
Human DNA sequence: ATATCGTAATGTTGGTG..500 more nucleotides....GTGTCAGGACGGGTGAAAGCCAGGACGCCGAATCG...5000 more nucleotides.
The underlined DNA signifies the gene of interest were trying to clone restriction enzyme sequences:
ERA I GTAATG
CRO I CAGGAC
MEM II GCCG
The number of restriction sites appear in the bacterial plasmid sequence is :

• A. More than 3
• B. 1
• C. 2
• D. 3
• E. None of the above

Answer 1

The correct option is C 2

The CRO I restriction sequence is found in both the human DNA and bacterial plasmid. The CAGGAC is found in both the genomes sequences and thus can be used to cut in both genomes before ligation.

So the correct answer is "2".