Question

Read the passage and answer the following question.

Bacterial plasmid sequence: ATCCCA...1000 more nucleotides..... ..ATTAAGGCCTTACAGGACCCCGGAATGAGAGTA ATGGGCTATTCAGGTA... 2000 more nucleotides
Human DNA sequence: ATATCGTAATGTTGGTG..500 more nucleotides.. ..GTGTCAGGACGGGTGAAAGCCAGGACGCCGAATCG...5000 more nucleotides
The underlined DNA signifies the gene of interest were trying to clone Restriction enzyme sequences:
ERA I GTAATG
CRO I CAGGAC
MEM II GCCG
Identify the correct enzyme(s) that should be used to insert the human sequence into the plasmid.

• A. ERA I
• B. CRO I
• C. MEM II
• D. CRO I and ERA I
• E. ERA I, CRO I, and MEM II

Answer 1

The correct option is A ERA I

ERA I has the sequence (GTAATG), which is present in the human DNA sequence. Hence, ERA I should be used to insert the human sequence into the plasmid so that it will cut on the human DNA as well as on the plasmid and hence, it will be easier to ligate as both will have sticky ends. Thus, the correct answer is option A.