Rearrange the following in the correct sequence to accomplish an important biological reaction-1- In vitro synthesis of copies of DNA of interest2- Chemically synthesized oligonucleotides3- Enzyme DNA-polymerase4- Complementary region of DNA5- Genomic DNA template6- Nucleotides provided7- Primers8- Thermostable DNA-polymerase from Thermus aquaticus9- Denaturation of ds-DNA

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Standard XII

Biology

Ligase

Rearrange the...

Question

Rearrange the following in the correct sequence to accomplish an important biological reaction:

In vitro synthesis of copies of DNA of interest

Chemically synthesized oligonucleotides

Enzyme DNA-polymerase

Complementary region of DNA

Genomic DNA template

Nucleotides provided

Primers

Thermostable DNA-polymerase (from Thermus aquaticus)

Denaturation of ds-DNA

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Solution

The correct sequence of the steps involved in biotechnological reaction are

Genomic DNA template

$↓$

Denaturation of ds-DNA

$↓$

Nucleotides provided

$↓$

Primers

$↓$

Thermostable DNA polymerase (from Thermus aquaticus)

$↓$

Enzyme DNA polymerase

$↓$

Chemically synthesised oligonucleotides

$↓$

Complementary region of DNA

$↓$

In vitro synthesis of copies of DNA of interest

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Similar questions

Q. Rearrange the following in the correct sequence to accomplish an important biotechnological reaction: [3]
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

Q. Rearrange the following in the current sequences to accomplish an important biotechnological reaction.
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

Q. Use of a thermostable DNA polymerase from the bacterium, Thermus aquatics, made it possible to generate a billion copies of DNA in a very short time using a process. Name the process.

Match the following enzymes of DNA replication with their respective functions:
$\begin{matrix} p . D N A p o l y m e r a s e & i . H e l p s u n z i p t h e D N A d o u b l e h e l i x q . D N A l i g a s e & i i . S e a l s n i c k s b e t w e e n O k a z a k i f r a g m e n t s r . D N A h e l i c a s e & i i i . L a y s R N A p r i m e r s t o w h i c h p o l y m e r a s e c a n a d d n u c l e o t i d e s s . P r i m a s e & i v . A d d s n u c l e o t i d e s t o t h e f r e e 3^{'} e n d o f R N A p r i m e r s \end{matrix}$

Match the following enzymes of DNA replication with their respective functions:

p. DNA polymerase i. Helps unzip the DNA double helix

q. DNA ligase ii. Seals nicks between Okazaki fragments

r. DNA helicase iii. Lays RNA primers to which polymerase can add nucleotides

s. Primase iv. Adds nucleotides to the free 3’ end of RNA primers

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