Rearrange the following in the correct sequence to accomplish an important biotechnological reaction: [3]
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

Open in App

Solution

(i) Denaturation of ds-DNA
(e) Genomic DNA template
(g) Primers
[1]Mark
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
[1]Mark

(f) Nucleotides provided
(d) Complementary region of DNA
(a) In vitro synthesis of copies of DNA of interest
[1]Mark

The above steps describe the process of Polymerase Chain Reaction (PCR).

No marks should be given if sequence is wrong even at one place.

Suggest Corrections

Similar questions

Q. Rearrange the following in the current sequences to accomplish an important biotechnological reaction.
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

Rearrange the following in the correct sequence to accomplish an important biological reaction:

Q. Use of a thermostable DNA polymerase from the bacterium, Thermus aquatics, made it possible to generate a billion copies of DNA in a very short time using a process. Name the process.

Q. A biology student is performing PCR for the first time. She adds the right DNA fragments and its corresponding primers. She also adds DNA polymerase and free nucleotides. The reaction mixture is kept in a water bath that measures

37^{\circ} C

. But DNA amplification does not occur. Why?

Match the following enzymes of DNA replication with their respective functions:
$\begin{matrix} p . D N A p o l y m e r a s e & i . H e l p s u n z i p t h e D N A d o u b l e h e l i x q . D N A l i g a s e & i i . S e a l s n i c k s b e t w e e n O k a z a k i f r a g m e n t s r . D N A h e l i c a s e & i i i . L a y s R N A p r i m e r s t o w h i c h p o l y m e r a s e c a n a d d n u c l e o t i d e s s . P r i m a s e & i v . A d d s n u c l e o t i d e s t o t h e f r e e 3^{'} e n d o f R N A p r i m e r s \end{matrix}$

Join BYJU'S Learning Program