Question

Rearrange the following in the correct sequence to accomplish an important biotechnological reaction: [3]
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

Answer 1

(i) Denaturation of ds-DNA
(e) Genomic DNA template
(g) Primers
[1]Mark
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
[1]Mark

(f) Nucleotides provided
(d) Complementary region of DNA
(a) In vitro synthesis of copies of DNA of interest
[1]Mark

The above steps describe the process of Polymerase Chain Reaction (PCR).

No marks should be given if sequence is wrong even at one place.