Question

Rearrange the following in the current sequences to accomplish an important biotechnological reaction.
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

Answer 1

The correct sequence of the steps involved in biotechnological reaction is

• Genomic DNA template
• Denaturation of ds-DNA
• Primers
• Nucleotides provided
• Thermostable DNA polymerase (from Thermus aquaticus)
  
• Enzyme DNA polymerase
• Chemically synthesised oligonucleotides
• Complementary region of DNA
• In vitro synthesis of copies of DNA of interest