MyQuestionIcon

1

You visited us 1 times! Enjoying our articles? Unlock Full Access!

Applications of Biotechnology

Recognition s...

Question

Recognition site plasmid having 2 Kbp at 500 bp, 800 bp, 1300 bp, 2000 bp
How many bands are visualise after gel electrophoresis after treating with R.E.

A

2

Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses

B

3

No worries! We‘ve got your back. Try BYJU‘S free classes today!

C

1

No worries! We‘ve got your back. Try BYJU‘S free classes today!

D

4

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Open in App

Solution

The correct option is A 2
As the restriction enzyme has a recognition site at 2Kb, after gel electrophoresis of the digested sample, two DNA fragments would be obtained.
So, the correct answer is '2'.

Suggest Corrections

thumbs-up

0

Similar questions

Q. A circular plasmid of

10, 000

base pairs (bp) is digested with two restriction enzymes, A and B, to produce a

3000

2000

bp bands when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at

5000

bp. If the first site for enzyme A(

A 1

) is present at the

100^{t h}

base, the order in which the remaining sites (

A 2, B 1

B 2

) are present is?

Q. Linear DNA fragments of sizes of 1500 bp, 3000 bp, 4000 bp and 4500 bp are obtained from gel electrophoresis. The position of these fragments from cathode to anode of the gel would be:

Q. As indicated in the gel image, lanes X and Y represent samples obtained from a circular plasmid DNA after complete digestion using restriction enzyme X or Y with different recognition sites, respectively. How many sites for X and Y are present in the plasmid (sizes of the bands in kilo base pairs(kb) is shown)?

Q. How are the following used in biotechnology ?

(a) plasmid DNA (b) recognition sequence (c) gel electrophoresis

Q. Eco RI restriction endonuclease requires a 6 bp sequence for recognition and cleavage. In a 10kb DNA fragment how many probable cleavage sites are present for this enzyme?

View More

Join BYJU'S Learning Program

similar_icon

Related Videos

lock

Biotechnology and Its Application

BIOLOGY

Watch in App

explore_more_icon

Explore more

Applications of Biotechnology

Standard X Biology

Join BYJU'S Learning Program

CrossIcon