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Applications of Biotechnology

Recognition s...

Question

Recognition sites for commonly used restriction enzymes meaning

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Solution

A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within the molecule known as restriction sites. ... Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially.

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Q. Which among the following are features required in a vector to facilitate cloning?
I. It should have ori - a sequence from where replication starts.
II. It should have a selectable marker.
III. It should have several recognition sites for a particular restriction enzyme.
IV. It should have a single recognition site for commonly used restriction enzymes.

Q. Assertion :Recognition site should be preferably single and responsive to commonly used restriction enzymes. Reason: In

p B R 322

D N A

is ligated generally in the area of

B a m - H I

site of tetracycline resistance gene.

Q. Assertion :The cloning vector is required to have very few, preferably single recognition commonly used used restriction enzymes. Reason: Presence of more than one recognition site within a cloning vector will generate which will complicate the process of gene cloning.

Q. Assertion :Recognition site should be preferably single and responsive to commonly used restriction enzyme. Reason: In pBR322 alien DNA is ligated generally in the area of Bam HI site of tetracyline resistance gene.

Q. Which one of the following is a recognition site for restriction enzyme Bam HI?

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