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Question

Restriction endonuclease, an enzyme used in genetic engineering is employed for

A
Probing exons
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B
Cutting double stranded DNA
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C
Cutting single stranded DNA
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D
Joining strands of DNA
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E
Copying DNA strands
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Solution

The correct option is B Cutting double stranded DNA
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e., each strand) of the DNA double helix. These enzymes are found in bacteria and archaea and provide a defence mechanism against invading viruses. Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called as restriction.

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