Question

Restriction endonuclease cuts DNA at specific sites and cellular DNA is not damaged as

• A. Rest enzyme susceptible sites are coated with protein
• B. Rest enzyme susceptible sites are catalyzed by particular enzymes
• C. They cleave DNA only at very limited and specific sites
• D. All of the above

Answer 1

The correct option is C They cleave DNA only at very limited and specific sites

The restriction enzymes used in molecular biology usually recognize short target sequences of about 4 8 base pairs. Restriction enzymes are very specific to their cleavage function. The site which is recognized by this enzyme for cleavage is the restriction site. Typically, a restriction site will be a palindromic sequence of about four to six nucleotides long where restriction endonuclease makes nick.

Therefore, the correct answer is option C.