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Competitive Inhibition

Restriction e...

Question

Restriction endonuclease Hind II always cuts DNA molecule at a particular point by recognising a specific sequence of

A

Six base pairs

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B

Five base pairs

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C

Four base pairs

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D

Seven base pairs

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E

Three base pairs

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Solution

The correct option is A Six base pairs
Hind II was the first discovered restriction endonuclease enzyme. It has been isolated from Haemophilus influenzae. It cuts DNA molecules at a particular point by recognising a specific sequence of six base pairs. This specific sequence is known as the recognition sequence for Hind II.
So the correct answer is option A.

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Q. First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is:

Q. Which of the following statements are correct?
(i) Restriction enzymes may cut the DNA strand a little away from the centre of the palindromic sequence, but between the same two bases on the opposite strands.
(ii) Hind ll always cuts DNA molecules at a particular site by recognising a specific sequence of six base pairs.
(iii) DNA is a positively charged molecule.

Q. Which of the following statements are correct?

(i) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site, but between the same two bases on the opposite strands.

(ii) Hind II always cuts DNA molecules at a particular point by recognising a specific sequence of six base pairs.

(iii) Separated DNA fragments cannot be visualised without staining on an agarose gel electrophoresis.

(iv) Ori is the sequence responsible for controlling the copy number.

(v) DNA is a positively charged molecule.

Q.
(a)EcoRI is a restriction endonuclease. How is it named so? Explain (b) Write the sequence of DNA bases that the enzyme recognises. Mention the point at which the enzyme makes a cut in the DNA segment.

Q. Each restriction enzyme recognises a specific base sequence known as . This site is in sequence.

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