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Question
Restriction endonuclease is employed for cutting
Restriction endonuclease is employed for cutting
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Solution
The correct option is C Double stranded DNA
Restriction endonuclease
Restriction endonucleases are enzymes which cleave the DNA by digesting phosphodiester bonds at specific sequences (recognition sequence) within the DNA. Examples include Hind III, EcoR I etc.
Nuclease
Any enzyme that helps in the cleavage of nucleic acids is called nucleases. It includes RNase (digest RNA) and DNase (digest DNA).
Limitation of REN
Restriction endonuclease cannot cleave RNA. It targets mainly DNA (double stranded or single stranded). It can also target RNA/DNA heteroduplexes.
Final answer
(B) Double stranded DNA
Restriction endonuclease
Restriction endonucleases are enzymes which cleave the DNA by digesting phosphodiester bonds at specific sequences (recognition sequence) within the DNA. Examples include Hind III, EcoR I etc.
Nuclease
Any enzyme that helps in the cleavage of nucleic acids is called nucleases. It includes RNase (digest RNA) and DNase (digest DNA).
Limitation of REN
Restriction endonuclease cannot cleave RNA. It targets mainly DNA (double stranded or single stranded). It can also target RNA/DNA heteroduplexes.
Final answer
(B) Double stranded DNA
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