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Question

Restriction endonucleases are enzymes that cleave DNA molecules into smaller fragments. Which type of bond do they act on?

A
N-glycosidic bond
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B
Phosphodiester bond
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C
Hydrogen bond
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D
Disulfide bond
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Solution

The correct option is B Phosphodiester bond
Restriction enzymes are the endonucleases which cleave the DNA at specific sites. These enzymes act on the phosphodiester bond which attaches two nucleotides in the polynucleotide chain. There are specific restriction sites at which the enzymes make blunt or staggered cuts to produce the fragments of the DNA.
Thus, the correct answer is option B.

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