Restriction endonucleases hydrolyses polynucleotide at

a random internal phosphodiester bond

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a phosphodiester bond within a specific sequence

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either terminal

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only the 5’ end.

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Solution

The correct option is B a phosphodiester bond within a specific sequence
Restriction endonuclease is an enzyme produced chiefly by certain bacteria. This enzyme cleaves the phosphodiester bond within or near a specific base sequence.

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Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

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