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B
1966
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C
1950
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D
1970
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Solution
The correct option is D 1970 Restriction endonucleases are the special types of nucleases that cleave the DNA internally at the specific sites called the restriction sites. These were discovered in 1970 by Hamilton O. Smith, Thomas Kelly and Kent Wilcox isolated from the bacterium Haemophilus influenzae. These enzymes are present in the bacterial cells as they cleave the DNA of any bacteriophage that happens to infect the bacterium. Due to their specific cleaving activity, they are extensively used in the rDNA processes.