Question

Restriction endonucleases were used by Cohen and Boyer's DNA experiments for

• A. Isolation of cloned bacterial plasmids
• B. Cleaving the bacterial plasmid
• C. Isolation of human insulin
• D. Both A and B

Answer 1

The correct option is D Both A and B

Stanley Cohen and Herbert Boyer used techniques to cut and paste DNA to create the first modified organism containing recombinant DNA. They inserted the rDNA molecule into E. coli cells through a plasmid by inducing uptake and expression of the foreign DNA known as transformation. Restriction enzymes can recognise specific DNA base pairs and cleave at a specific site. Restriction enzymes are used to cleave the bacterial plasmid and the desired DNA segment. These enzymes can be used in the isolation and cleaving of the plasmids which are used as the vectors.

Thus, the correct answer is option D.