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Restriction enzymes are isolated chiefly from
Restriction enzymes are isolated chiefly from
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The correct option is D Prokaryotes
Restriction endonucleases are enzymes, isolated chiefly from prokaryotes, that recognize specific nucleotide sequences within double stranded DNA and cleaves it at these sites. These restriction endonucleases facilitate the specific fragmentation of double stranded DNA and are very useful for DNA sequence analysis. These enzymes can be classified into three groups. Type I and type III enzymes carry a modification (methylation) and an ATP requiring restriction (cleavage) activity within the same protein. Both types of enzymes identify unmethylated sequences in substrate DNA, but type I enzymes cleave randomly, whereas type III enzymes cut DNA at specific sites. Type II restriction enzyme consist of a separate restriction endonuclease and a modification methylase.
Restriction endonucleases are enzymes, isolated chiefly from prokaryotes, that recognize specific nucleotide sequences within double stranded DNA and cleaves it at these sites. These restriction endonucleases facilitate the specific fragmentation of double stranded DNA and are very useful for DNA sequence analysis. These enzymes can be classified into three groups. Type I and type III enzymes carry a modification (methylation) and an ATP requiring restriction (cleavage) activity within the same protein. Both types of enzymes identify unmethylated sequences in substrate DNA, but type I enzymes cleave randomly, whereas type III enzymes cut DNA at specific sites. Type II restriction enzyme consist of a separate restriction endonuclease and a modification methylase.
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