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Restriction enzymes were discovered by microbiologists who were studying the host range of infectious bacteriophage. The term restriction refers to the fact that different strains of bacteria are resistant to bacteriophage infection if they contain the appropriate endonucleases that can destroy infecting DNA. Observe this figure and answer the following questions.

Can you pick up from figure, how the bacteria protects its own genome from degradation by restriction endonucleases?

223100_9a0d10b22bdf4a1e90620aa1e17037b0.png

A
Site specific coupling of its own DNA
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B
Site specific oxidation of its own DNA
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C
Site specific methylation of its own DNA
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D
All of the above
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Solution

The correct option is C Site specific methylation of its own DNA
  • Restriction enzymes cut foreign DNA, such as viral DNA, into fragments.
  • Bacteria protect their own DNA by modifying bases, usually by methylation, at the recognition sites.
  • Hence, the bacteria protects its own genome from degradation by restriction endonucleases by Site-specific methylation of its own DNA.
  • So, the correct answer is 'Site-specific methylation of its own DNA'.

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