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Question

Restriction enzymes were used to cleave a section of DNA into four fragments. These fragments were 400, 600, 1800, and 3000 base pairs long.
Which gel electrophoresis well in the image most likely represents these fragments?
Note: The wells are drawn as boxes at the bottom of the diagram.
529862.PNG

A
Well A
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B
Well B
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C
Well C
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D
Well D
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Solution

The correct option is A Well A
DNA fragments when subjected to gel electrophoresis move towards anode or the positive electrode. The speed and distance of movement depends upon the length of the DNA fragment that is also the number of base pairs that make the fragment. So, the shorter fragments will travel faster and farther from the wells as compared to the larger fragments. Based on the number of base pairs the image of well A represents the distribution
So, the correct answer is 'Well A'

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