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Byju's Answer
Standard VII
Biology
Blunt and Sticky Ends
"Restriction"...
Question
"Restriction" in Restriction enzyme refers to
A
Cleaving of phosphodiester bond in DNA by the enzyme
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B
Cutting of DNA at specific position only
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C
Prevention of the multiplication of bacteriophage in bacteria
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D
Restriction of bacterial multiplication
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Solution
The correct option is
B
Cutting of DNA at specific position only
Enzyme involve in the cutting of dsDNA molecule at palindromic sites are called Restriction endonucleases.
Examples are EcoR I, BamH I, Hind III, Pst I, Sal I.
So, the correct option is 'cutting of DNA at specific position only'.
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Similar questions
Q.
Read the following statements and select the correct ones.
(i) Same kind of sticky ends are produced when a DNA is cut by different restriction enzymes.
(ii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind II was the first restriction endonuclease to be isolated.
(iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) Presence of more than one recognition site for the same restriction enzyme within the vector complicates gene cloning.
Q.
Read the following statements and select the correct ones.
(i) The same kind of sticky ends are produced when DNA has been cut by different restriction enzymes.
(ii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind II was the first restriction endonuclease to be isolated.
(iv) A bacteriophage can replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) The presence of more than one recognition site for an enzyme within the vector complicates the gene cloning.
Q.
Read the following statements and choose the appropriate option.
Statement 1 - The enzyme catalysing the removal of nucleotides from the ends of DNA is exonuclease.
Statement 2 - The enzyme that cleaves phosphodiester bonds at any site within the DNA is exonuclease.
Statement 3 - The enzyme that cleaves phosphodiester bonds at specific sites within the DNA is endonuclease.
Q.
Restriction enzymes can cleave the DNA at any site randomly.
Q.
Bacterial DNA protects its own DNA from being cut up by the restriction enzymes present in the cell because _____________ .
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Blunt and Sticky Ends
Standard VII Biology
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