Question

Restriction sites for Pvu I and Pvu II respectively are in which genes of pBR 322

• A. $rop$, $am{p}^R$
• B. $am{p}^R$, $rop$
• C. $rop$, $ori$
• D. $ori$, $rop$

Answer 1

Answer: (B)

$am{p}^R$, $rop$

Restriction sites for Pvu I and Pvu II for pBR322 are present on the amp${}^R$ and rop gene sites of the plasmid. The amp${}^R$ is the ampicillin resistance site which is used as a selectable marker. The rop gene codes for rop protein in the vector.

So, the correct answer is option B.