Question

RNA interference which is employed in making tobacco plant resistant to Meloidegyne incognita is essentially involved in ?

• A. Preventing the process of translation of mRNA
• B. Preventing the process of transcription
• C. Preventing the process of replication of DNA
• D. Preventing the process of splicing of hnRNA

Answer 1

The correct option is A Preventing the process of translation of mRNA

RNA interference method involves the participation of a long double-stranded RNA (dsRNA) to silence the expression of target genes. Upon introduction, the dsRNA enters a cellular pathway, gets processed into small interfering RNA (siRNA) then siRNA assemble into RNA-induced silencing complex. The siRNA subsequently guide the complex to complementary RNA molecules where they cleave and destroy the target mRNA. In this way, the entire process causes silencing of mRNA. Meloidogyne incognita infects the roots of tobacco plants and causes a great loss by causing the reduction in yield. Because of RNAi, these plants are made resistant to this nematode.

Thus, the correct answer is option A.