253
Question
Safranin stains which element of the tissues?
Safranin stains which element of the tissues?
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Solution
The correct option is B Lignified elements
Safranin O is a regressive dye and requires destaining and differentiation with picric acid or HCl. The Safranin-stained tissues are counterstained with Fast Green — a progressive dye. At the concentration used, Fast Green stains the tissues within 10–15 s, so it is best to test the procedure on one or two slides before staining your entire set. In plant tissues stained with this method, Safranin O appears brilliant red in chromosomes, nuclei, lignified, suberized, or cutinized cell walls. Fast Green appears a brilliant green in cytoplasm and cellulosic cell walls. Fast Green turns blue in basic solutions. It appears blue to bluish-green in the stems and leaves of aquatic plants and most gymnosperms. An aqueous alternative to Fast Green FCF is Alcian Blue. Safranin does not stains starch, based and protein elements.
Therefore, the correct answer is option B.
Safranin O is a regressive dye and requires destaining and differentiation with picric acid or HCl. The Safranin-stained tissues are counterstained with Fast Green — a progressive dye. At the concentration used, Fast Green stains the tissues within 10–15 s, so it is best to test the procedure on one or two slides before staining your entire set. In plant tissues stained with this method, Safranin O appears brilliant red in chromosomes, nuclei, lignified, suberized, or cutinized cell walls. Fast Green appears a brilliant green in cytoplasm and cellulosic cell walls. Fast Green turns blue in basic solutions. It appears blue to bluish-green in the stems and leaves of aquatic plants and most gymnosperms. An aqueous alternative to Fast Green FCF is Alcian Blue. Safranin does not stains starch, based and protein elements.
Therefore, the correct answer is option B.
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