MyQuestionIcon

1

You visited us 1 times! Enjoying our articles? Unlock Full Access!

What Is a Gene?

Screening the...

Question

Screening the fragments for a 'desired gene'.Explain.

Open in App

Solution

The term "Screening the fragments of desired gene" means that we need to selected a gene of our desire which has all the characters needed for the experiment. From the genes isolated, we need to screen them according to our needs.

Suggest Corrections

thumbs-up

0

Similar questions

Q. When genomic DNA is fragmented and cloned, the screening of the desired gene is done by using ?

Q. Any recombinant DNA with a desired gene is required in billion copies for commercial use. How is the amplification done? Explain.

Q. The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps ?

Q. Study the following lists.
List-I List-II
(A) Colony hybridization (I) Transfer of recombinant DNA into a host cell
(B) Gel electrophoresis (II) Selection of cells containing the desired gene
(C) Gradient centrifugation (III) Separation of DNA fragments
(D) Polymerase chain reaction (IV) Purification of DNA
(V) Gene cloning in thermocycler
The correct match is

Q. This is figure of plasmid pBR 322. The gene conferring resistance to ampicillin (ApR) can be interrupted by insertion of a DNA fragment into the Pstl site, and the gene conferring resistance to tetracycline (TcR) can be interrupted by insertion of a DNA fragment into the BamHI site. Replication is controlled by the CoIEl origin. Use of the TcR and ApR genes allows for easy screening for recombinants carrying inserts of foreign DNA. For locating Eco RI, TcR and ApR gene on it. Identify, what is represented by A, B and C.

View More

Join BYJU'S Learning Program

similar_icon

Related Videos

lock

What is a Gene?

BIOLOGY

Watch in App

explore_more_icon

Explore more

What Is a Gene?

Standard X Biology

Join BYJU'S Learning Program

CrossIcon