Question

Select the incorrect fact about given techniques :

• A. ELISA - alkaline phosphatase and peroxidase enzymes are used
• B. FNAC - Used for detection for breast tumor
• C. Pap Smear - Used for cervical carcinoma detection
• D. PTCA - A blood vessel from leg or chest is used to by pass the blocked region of coronary artery

Answer 1

Answer: (A), (C)

The correct options are
A ELISA - alkaline phosphatase and peroxidase enzymes are used
C Pap Smear - Used for cervical carcinoma detection

ELISA (enzyme-linked immunosorbent assay) is also called as EIA (enzyme immunoassay). This technique is used to detect and quantify substances like proteins, antibodies, hormones. In this, alkaline phosphatase (ALP) and horseradish peroxidase (HRP) enzymes are used. Both these enzymes require different substrates. High purified IgG are usually used in conjugate with enzyme. Fine Needle Aspiration Cytology (FNAC) is used to detect lump in superficial areas. Pap Smear is used to detect cervical cancer. Percutaneous transluminal coronary angioplasty (PTCA) is a technique used to open up blocked coronary arteries.

Thus, the correct answer is 'ELISA - alkaline phosphatase and peroxidase enzymes are used and Pap Smear - Used for cervical carcinoma detection.'