3
Question
Select the option that correctly identifies the given statements as true or false.
I. The instrument used for PCR is called thermocycler.
II. The copies of DNA synthesised by PCR are not identical and each copy formed has certain variations.
III. PCR amplifies DNA strands at an exponential rate.
Select the option that correctly identifies the given statements as true or false.
I. The instrument used for PCR is called thermocycler.
II. The copies of DNA synthesised by PCR are not identical and each copy formed has certain variations.
III. PCR amplifies DNA strands at an exponential rate.
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Solution
The correct option is A I - T, II - F, III - T
PCR or polymerase chain reaction is a technique used in molecular biology to create several identical copies of a certain DNA segment.
A thermocycler or DNA amplifier is a laboratory device used to amplify small samples of DNA via polymerase chain reaction.
PCR needs the reaction mixture to be taken through different levels of temperature very quickly for a successful amplification. This is done by using a thermocycler that can easily switch the reaction conditions between different temperatures at accurate time intervals.
The number of DNA molecules is doubled in each cycle of PCR.
The formula to calculate the number of DNA molecules formed after a given number of cycles is 2n (n = number of cycles of PCR).
For example, if we were to do 32 rounds of PCR, the number of molecules of double-stranded DNA would be 232 . The value will reach up to billions. Hence, it can be concluded that PCR amplifies DNA at an exponential rate.
PCR or polymerase chain reaction is a technique used in molecular biology to create several identical copies of a certain DNA segment.
A thermocycler or DNA amplifier is a laboratory device used to amplify small samples of DNA via polymerase chain reaction.
PCR needs the reaction mixture to be taken through different levels of temperature very quickly for a successful amplification. This is done by using a thermocycler that can easily switch the reaction conditions between different temperatures at accurate time intervals.
The number of DNA molecules is doubled in each cycle of PCR.
The formula to calculate the number of DNA molecules formed after a given number of cycles is 2n (n = number of cycles of PCR).
For example, if we were to do 32 rounds of PCR, the number of molecules of double-stranded DNA would be 232 . The value will reach up to billions. Hence, it can be concluded that PCR amplifies DNA at an exponential rate.
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