1
Question
Selectable markers in plasmids are used for
Selectable markers in plasmids are used for
Open in App
Solution
The correct option is A Identifying recombinants from non-recombinants
Plasmid
Plasmids are extranuclear, circular, double stranded DNA. They are usually used as vectors in genetic engineering.
Selectable marker
Selectable marker is a gene in a vector that helps in the selection of transformants or recombinants (host cells which have successfully taken up the foreign DNA) and non-transformants or non-recombinants (cells which have not taken up the vector DNA).
For example, antibiotic resistance genes in the vector.
Antibiotic resistance gene as selectable marker
An antibiotic resistance gene in a plasmid vector helps in the selection of transformants in the following way:
● The plasmid containing antibiotic resistance gene as selectable markers enters the competent host cell where multiple copies of the plasmids can be made.
● If the host cell gains resistance to a particular antibiotic then it indicates that transformation of the host cell with the plasmid is successful.
● Thus the presence of the selectable marker helps in identifying and eliminating non-transformants or non recombinant cells and selectively permitting the growth of transformants on a media containing the antibiotic.
Cancer biomarkers
The biological molecules produced by the body or tumor in a person with cancer are called cancer biomarkers. Examples include DNA, RNA, protein etc.
Microbiological assays
The presence of antibiotics can be detected by microbiological assays where the antibiotic-sensitive species of bacteria are used to determine the presence of specific antibiotics in a given sample.
Final answer
(C) Identifying recombinants from non-recombinants
Plasmid
Plasmids are extranuclear, circular, double stranded DNA. They are usually used as vectors in genetic engineering.
Selectable marker
Selectable marker is a gene in a vector that helps in the selection of transformants or recombinants (host cells which have successfully taken up the foreign DNA) and non-transformants or non-recombinants (cells which have not taken up the vector DNA).
For example, antibiotic resistance genes in the vector.
Antibiotic resistance gene as selectable marker
An antibiotic resistance gene in a plasmid vector helps in the selection of transformants in the following way:
● The plasmid containing antibiotic resistance gene as selectable markers enters the competent host cell where multiple copies of the plasmids can be made.
● If the host cell gains resistance to a particular antibiotic then it indicates that transformation of the host cell with the plasmid is successful.
● Thus the presence of the selectable marker helps in identifying and eliminating non-transformants or non recombinant cells and selectively permitting the growth of transformants on a media containing the antibiotic.
Cancer biomarkers
The biological molecules produced by the body or tumor in a person with cancer are called cancer biomarkers. Examples include DNA, RNA, protein etc.
Microbiological assays
The presence of antibiotics can be detected by microbiological assays where the antibiotic-sensitive species of bacteria are used to determine the presence of specific antibiotics in a given sample.
Final answer
(C) Identifying recombinants from non-recombinants
Suggest Corrections
0
Similar questions
View More
Join BYJU'S Learning Program
Explore more
Join BYJU'S Learning Program
