Question

Separation and purification by filtration, centrifugation of the desired compound produced in bioreactor is a part of

• A. downstream processing only
• B. scaling up and downstream processing
• C. upstream processing
• D. screening for recombinants and downstream processing

Answer 1

The correct option is A downstream processing only

Bioreactors are vessels in which raw materials are biologically converted into specific products, individual enzymes, etc. using microbial plant, animal or human cells.

Before marketing it as a finished product, they are passed through downstream processing. The processes include separation and purification by filtration and centrifugation.

Upstream Processing refers to the first step in which biomolecules are grown in bioreactors, usually by bacterial or mammalian cell lines.

Scaling up and downstream processing focuses on the developmental work in scaling-up of fermentation processes and downstream processing for the purpose of separation, purification, and polishing of bioproducts.

Screening for recombinants and downstream processing is required after insertion of recombinant DNA into the host cell.