Shown above is the structure of a Drosophila gene, divided into 10 segments and designated A-J. The gene contains three exons, two introns, a promoter and a site in I for poly (A) addition. What segment or the segments of the gene contains CCAAT box and TATA box elements?

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Both A and B

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Solution

The correct option is A Both A and B
TATA box is located 20 bp upstream the start site and has a consensus sequence as “TATAA/TAA/T”. CAAT box is located 70-80 bp upstream the start site and has consensus sequence as “GGT/ACAATCT”. This means that CAAT and TATA boxes can be present in A, B or C blocks which are present upstream the first exon. Since D block has first exon, the start site must be present in this block; thus, it can’t have CAAT and TATA boxes which are present upstream the start site. Correct options are A and B.

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