Question

Significance of heat shock method in bacterial transformation is to facilitate

• A. binding of DNA to the cell wall
• B. uptake of DNA through membrane transport proteins
• C. uptake of DNA through transient pores in the bacterial cell wall
• D. expression of antibiotic resistance gene

Answer 1

The correct option is C uptake of DNA through transient pores in the bacterial cell wall

In heat shock method, the bacterial cells are incubated with recombinant DNA on ice, followed by placing them briefly at 42${}^o$ C and then putting it back on ice. The bacteria now takes up the recombinant DNA through transient pores in the bacterial cell wall.