Question

Significance of the ‘heat shock’ in bacterial transformation is to facilitate

Answer 1

Process of heat shock method is as follows:

• Bacterial cells are treated with divalent ions $\left(C{a}^{2+}\right)$.
• The competent bacterial cells are incubated on ice along with $rDNA$. Then a heat shock at ${42}^{\circ }C$ is provided for a brief time period which creates transient pores within the bacterial cell membrane.
• These pores allow the $DNA$ of interest to enter into the bacteria.
• These cells are placed back on ice to retain the plasmids inside the bacteria.