Question

Small chemically synthesised oligonucleotides that are complementary to the regions of DNA are called primers and present at the

• A. 5’ end
• B. 3’ end
• C. both 3’ and 5’ end
• D. middle of 3' and 5' end

Answer 1

The correct option is B 3’ end

Primers are small chemically synthesised oligonucleotides that are complementary to the sequences present at 3′ end of the template DNA. Primers are a short single-stranded nucleic acid used by all living organisms in the initiation of DNA synthesis. They hybridise to the target DNA region, one to each strand of the double helix.