Question

Small chemically synthesised oligonucleotides that are complementary to the regions of DNA are called primers and present at the ______ .

• A. 3’end
• B. None of the above
• C. Both 3’ and 5’ end
• D. 5’end

Answer 1

The correct option is A 3’end

Annealing

Annealing involves hybridisation of the primers with specific sequences of each strand of the template DNA at the 3’end . Primers are small oligonucleotides up to 18 - 24 nucleotides long synthesised chemically. This process is carried out at 50 - 56°C.

Final answer

(b) 3’ end