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Question
Some of the steps involved in gene cloning are given below. Arrange them in the correct order.
I. Introduction of the identified DNA into the host.
II. Selection of hosts having desired DNA.
III. Identification of DNA with desirable genes.
IV. Transfer of the desired DNA to its progeny.
Some of the steps involved in gene cloning are given below. Arrange them in the correct order.
I. Introduction of the identified DNA into the host.
II. Selection of hosts having desired DNA.
III. Identification of DNA with desirable genes.
IV. Transfer of the desired DNA to its progeny.
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Solution
The correct option is C III, I, II, IV
The techniques of genetic engineering used to create exact multiple copies of a desired gene is called gene cloning or DNA cloning.
It is done by identifying the desired gene in the DNA followed by digestion with suitable restriction enzymes (DNA cutting enzymes). This yields DNA fragments with the gene of interest. It is then introduced into a host. The host with foreign DNA inserts is selected. These are further grown and allowed to transfer the desired DNA to the progeny by repeated multiplication.
Steps involved in gene cloning:
Identification of DNA with desirable genes → Introduction of the identified DNA into the host → Selection of hosts having desired DNA → Transfer of the desired DNA to its progeny.
The techniques of genetic engineering used to create exact multiple copies of a desired gene is called gene cloning or DNA cloning.
It is done by identifying the desired gene in the DNA followed by digestion with suitable restriction enzymes (DNA cutting enzymes). This yields DNA fragments with the gene of interest. It is then introduced into a host. The host with foreign DNA inserts is selected. These are further grown and allowed to transfer the desired DNA to the progeny by repeated multiplication.
Steps involved in gene cloning:
Identification of DNA with desirable genes → Introduction of the identified DNA into the host → Selection of hosts having desired DNA → Transfer of the desired DNA to its progeny.
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