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Sparged stirred-tank bioreactors have been designed for
Sparged stirred-tank bioreactors have been designed for
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The correct option is D increased surface area for oxygen transfer into culture
Bioreactors are large vessels that are used for large scale culturing of host cells carrying the recombinant DNA (plant, animal or microbial) under controlled conditions (temperature, pH, nutrients, oxygen (aerobic bioreactors), etc.) to obtain specific gene products for commercial use.
One of the most commonly used bioreactors are stirred-tank type bioreactors. Aerobic stirred tank type bioreactors can further be of two types - simple stirred-tank bioreactors and sparged stirred-tank bioreactors.
Sparged stirred-tank bioreactors provide increased surface area for aeration of the culture broth. They provide a considerable surface area available where air above the liquid culture gets trapped in the form of bubbles which then travels throughout the culture. This is also known as gas entrainment. Besides, in these tanks, air bubbles are sparged in the medium with the help of a sparger. This dramatically increases the effective surface area available for oxygen transfer.
Once the concentration of the product expressed by the host cells in the culture reaches the optimal quantity, it is separated from the culture media and purified. This process is known as downstream processing.
The purified product is then formulated with preservatives to increase shelf life.
The biosynthesis of certain desired products requires the host cells to grow in the absence of oxygen, that is, in an anaerobic condition. In such cases, specially designed anaerobic bioreactors are used. Examples include production of fermented products, anaerobic digestion of sludge, etc.
Sparged stirred-tank bioreactor is not designed to ensure anaerobic conditions in the culture vessel.
Bioreactors are large vessels that are used for large scale culturing of host cells carrying the recombinant DNA (plant, animal or microbial) under controlled conditions (temperature, pH, nutrients, oxygen (aerobic bioreactors), etc.) to obtain specific gene products for commercial use.
One of the most commonly used bioreactors are stirred-tank type bioreactors. Aerobic stirred tank type bioreactors can further be of two types - simple stirred-tank bioreactors and sparged stirred-tank bioreactors.
Sparged stirred-tank bioreactors provide increased surface area for aeration of the culture broth. They provide a considerable surface area available where air above the liquid culture gets trapped in the form of bubbles which then travels throughout the culture. This is also known as gas entrainment. Besides, in these tanks, air bubbles are sparged in the medium with the help of a sparger. This dramatically increases the effective surface area available for oxygen transfer.
Once the concentration of the product expressed by the host cells in the culture reaches the optimal quantity, it is separated from the culture media and purified. This process is known as downstream processing.
The purified product is then formulated with preservatives to increase shelf life.
The biosynthesis of certain desired products requires the host cells to grow in the absence of oxygen, that is, in an anaerobic condition. In such cases, specially designed anaerobic bioreactors are used. Examples include production of fermented products, anaerobic digestion of sludge, etc.
Sparged stirred-tank bioreactor is not designed to ensure anaerobic conditions in the culture vessel.
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